杜洛克猪HLCS基因组织表达分析及其编码区多态性与剩余采食量的关联

doi:10.11843/j.issn.0366-6964.2018.06.002

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (6): 1108-1115.doi: 10.11843/j.issn.0366-6964.2018.06.002

杜洛克猪HLCS基因组织表达分析及其编码区多态性与剩余采食量的关联

张跃博¹, 蒲蕾², 张金山¹, 颜华¹, 王立刚¹, 侯欣华¹, 刘欣¹, 高红梅¹, 王立贤¹, 张龙超^1*

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 天津农学院动物科学与动物医学学院, 天津 300384

收稿日期:2017-12-28 出版日期:2018-06-23 发布日期:2018-06-23
通讯作者: 张龙超,副研究员,主要从事猪分子遗传育种研究,Tel:010-62816011,E-mail:zhlchias@163.com
作者简介:张跃博(1990-),男,山东武城人,博士生,主要从事猪分子遗传研究,Tel:010-62816011,E-mail:ybzhangfd@126.com
基金资助:
中国农业科学院科技创新工程（ASTIP-IAS02）；国家生猪产业技术体系（CARS-36）；中国农业科学院北京畜牧兽医研究所中央级公益性科研院所基本科研业务费专项资金项目（2017ywf-zd-12；2017ywf-zd-14；2017ywf-zd-22）；天津市科技计划项目（15ZXZYNC00100）；天津生猪产业技术体系创新团队（ITTPRS2017006）

Tissue Expression Profiles of HLCS Gene and Its Association with Residual Feed Intake in Duroc

ZHANG Yue-bo¹, PU Lei², ZHANG Jin-shan¹, YAN Hua¹, WANG Li-gang¹, HOU Xin-hua¹, LIU Xin¹, GAO Hong-mei¹, WANG Li-xian¹, ZHANG Long-chao^1*

1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2017-12-28 Online:2018-06-23 Published:2018-06-23
Supported by:

摘要/Abstract

摘要：

旨在探究羧化全酶合成酶基因（holocarboxylase synthetase，HLCS）在杜洛克猪各组织中的表达情况及其编码区多态性与剩余采食量（residual feed intake，RFI）之间的关系。本研究利用荧光定量PCR检测HLCS基因在杜洛克猪8种组织中的相对表达量；利用PCR和测序在RFI高、低组个体中扩增HLCS基因的编码区用以寻找多态位点，并对获得的位点在杜洛克群体中分型，进而分析多态位点与RFI的关联性。结果表明，HLCS在脂肪组织中表达量最高，肝次之，在心和肌肉中痕量表达。在杜洛克个体中共发现5个多态位点，其中c.1408G > C和c.1976A > G为错义突变位点。在杜洛克群体中，c.1408G > C与RFI关联不显著（P>0.05）；c.1976A > G与RFI显著关联（P<0.05），GG基因型个体的RFI比AA基因型个体低0.063 4 kg。这些结果表明，HLCS与RFI密切相关，c.1976A > G可作为猪RFI性状选育的潜在分子标记，为进一步开展HLCS基因影响猪剩余采食量的功能鉴定奠定了研究基础。

Abstract:

This experiment was conducted to explore the tissue expression profile of HLCS, the polymorphisms in its coding region and the association of different genotypes with residual feed intake in Duroc. Real-time PCR was used to detect HLCS mRNA expression in 8 tissues of Duroc pigs. The polymorphic sites of HLCS were determined in Duroc with high and low RFI lines by PCR and sequencing. Genotypes were detected in Duroc, and their associations with residual feed intake trait were analyzed. Real-time PCR showed that HLCS had the highest expression in backfat, followed by liver, and it had the lowest expression in heart and muscle. Five SNPs were detected in the coding region of HLCS gene. Two of them, c.1408G > C and c.1976A > G were nonsynonymous mutations. The site c.1976A > G was significantly associated with RFI(P<0.05), and the individuals with GG genotype had lower RFI than individuals with AA genotype. These results indicate that HLCS may play an important role in porcine RFI, and c.1976A > G can serve as a useful genetic marker for reducing RFI in swine. This study is valuable to further explore the molecular mechanism of HLCS affecting residual feed intake.

中图分类号:

S828.2

张跃博, 蒲蕾, 张金山, 颜华, 王立刚, 侯欣华, 刘欣, 高红梅, 王立贤, 张龙超. 杜洛克猪HLCS基因组织表达分析及其编码区多态性与剩余采食量的关联[J]. 畜牧兽医学报, 2018, 49(6): 1108-1115.

ZHANG Yue-bo, PU Lei, ZHANG Jin-shan, YAN Hua, WANG Li-gang, HOU Xin-hua, LIU Xin, GAO Hong-mei, WANG Li-xian, ZHANG Long-chao. Tissue Expression Profiles of HLCS Gene and Its Association with Residual Feed Intake in Duroc[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1108-1115.

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杜洛克猪HLCS基因组织表达分析及其编码区多态性与剩余采食量的关联

Tissue Expression Profiles of HLCS Gene and Its Association with Residual Feed Intake in Duroc

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